@article{87636,
  keywords = {Animals, Base Sequence, Drosophila, Gene Expression Regulation, Mutation, Molecular Sequence Data, Binding Sites, Transcription Factors, Phenotype, DNA-Binding Proteins, Enhancer Elements, Genetic, Drosophila Proteins},
  author = {Susan Schweinsberg and Kirsten Hagstrom and Daryl Gohl and Paul Schedl and Ram Kumar and Rakesh Mishra and Francois Karch},
  title = {The enhancer-blocking activity of the Fab-7 boundary from the Drosophila bithorax complex requires GAGA-factor-binding sites.},
  abstract = { In the work reported here we have analyzed the role of the GAGA factor [encoded by the Trithorax-like (Trl) gene] in the enhancer-blocking activity of Frontabdominal-7 (Fab-7), a domain boundary element from the Drosophila melanogaster bithorax complex (BX-C). One of the three nuclease hypersensitive sites in the Fab-7 boundary, HS1, contains multiple consensus-binding sequences for the GAGA factor, a protein known to be involved in the formation and/or maintenance of nucleosome-free regions of chromatin. GAGA protein has been shown to localize to the Fab-7 boundary in vivo, and we show that it recognizes sequences from HS1 in vitro. Using two different transgene assays we demonstrate that GAGA-factor-binding sites are necessary but not sufficient for full Fab-7 enhancer-blocking activity. We show that distinct GAGA sites are required for different enhancer-blocking activities at different stages of development. We also show that the enhancer-blocking activity of the endogenous Fab-7 boundary is sensitive to mutations in the gene encoding the GAGA factor Trithorax-like. },
  year = {2004},
  journal = {Genetics},
  volume = {168},
  pages = {1371-84},
  month = {11/2004},
  issn = {0016-6731},
  doi = {10.1534/genetics.104.029561},
  language = {eng},
}