@article{204011,
  keywords = {Animals, Drosophila, Transcription Factors, Protein Binding, Embryonic Development, Gene Expression Regulation, Developmental, Drosophila Proteins, Co-Repressor Proteins, Insulator Elements},
  author = {Anna Fedotova and Tsutomu Aoki and Mika{\"e}l Rossier and Rakesh Kumar Mishra and Chaevia Clendinen and Olga Kyrchanova and Daniel Wolle and Artem Bonchuk and Robert Maeda and Annick Mutero and Fabienne Cleard and Vladic Mogila and Fran{\c c}ois Karch and Pavel Georgiev and Paul Schedl},
  title = {The BEN Domain Protein Insensitive Binds to the  Chromatin Boundary To Establish Proper Segmental Identity in },
  abstract = { Boundaries (insulators) in the  bithorax complex (BX-C) delimit autonomous regulatory domains that orchestrate the parasegment (PS)-specific expression of the BX-C homeotic genes. The  boundary separates the  and  regulatory domains, which control Abd-B expression in PS11 and PS12, respectively. This boundary is composed of multiple functionally redundant elements and has two key functions: it blocks cross talk between  and  and facilitates boundary bypass. Here, we show that two BEN domain protein complexes, Insensitive and Elba, bind to multiple sequences located in the  nuclease hypersensitive regions. Two of these sequences are recognized by both Insv and Elba and correspond to a CCAATTGG palindrome. Elba also binds to a related CCAATAAG sequence, while Insv does not. However, the third Insv recognition sequences is \~{}100 bp in length and contains the CCAATAAG sequence at one end. Both Insv and Elba are assembled into large complexes (\~{}420 and \~{}265-290 kDa, respectively) in nuclear extracts. Using a sensitized genetic background, we show that the Insv protein is required for  boundary function and that PS11 identity is not properly established in  mutants. This is the first demonstration that a BEN domain protein is important for the functioning of an endogenous fly boundary. },
  year = {2018},
  journal = {Genetics},
  volume = {210},
  pages = {573-585},
  month = {10/2018},
  issn = {1943-2631},
  doi = {10.1534/genetics.118.301259},
  language = {eng},
}