@article{203971,
  keywords = {Animals, Mutation, RNA, Messenger, Homeostasis, Female, Microtubules, Oocytes, Drosophila melanogaster, Protein Transport, Drosophila Proteins, RNA-Binding Proteins, Ovary, 3{\textquoteright} Untranslated Regions, Synaptonemal Complex},
  author = {Justinn Barr and Rudolf Gilmutdinov and Linus Wang and Yulii Shidlovskii and Paul Schedl},
  title = {The  CPEB Protein Orb Specifies Oocyte Fate by a 3{\textquoteright}UTR-Dependent Autoregulatory Loop},
  abstract = {  encodes one of the two fly CPEB proteins. These widely conserved proteins bind to the 3{\textquoteright}UTRs of target messenger RNAs (mRNAs) and activate or repress their translation. We show here that a positive autoregulatory loop driven by the  gene propels the specification of oocyte identity in  egg chambers. Oocyte fate specification is mediated by a 3{\textquoteright}UTR-dependent mechanism that concentrates  mRNAs and proteins in one of the two pro-oocytes in the 16-cell germline cyst. When the  3{\textquoteright}UTR is deleted,  mRNA and protein fail to localize and all 16 cells become nurse cells. In wild type, the oocyte is specified when  and other gene products concentrate in a single cell in region 2b of the germarium. A partially functional  3{\textquoteright}UTR replacement delays oocyte specification until the egg chambers reach stage 2 of oogenesis. Before this point,  mRNA and protein are unlocalized, as are other markers of oocyte identity, and the oocyte is not specified. After stage 2, \~{}50\% of the chambers successfully localize  in a single cell, and this cell assumes oocyte identity. In the remaining chambers, the  autoregulatory loop is not activated and no oocyte is formed. Finally, maintenance of oocyte identity requires continuous  activity. },
  year = {2019},
  journal = {Genetics},
  volume = {213},
  pages = {1431-1446},
  month = {12/2019},
  issn = {1943-2631},
  doi = {10.1534/genetics.119.302687},
  language = {eng},
}